biographical sketch

Yinghua Zhang, MD, PhD Research Associate

education

Sun Yat-sen University of Medical Sciences, MD 1982 China

Kyoto Prefectural University of Medicine, Japan PhD 1998

Georgetown University Medical Center, USA Postdoc

1999-02

Research and Professional Experience:

1982-1988 Residency, Department of Obstetrics and Gynecology, Guangzhou Medical University Hospital, P.R. China

1988-1989 Research Fellow, Department of Hygiene, Kyoto Prefectural University of Medicine, Japan

1993-1998 Research Scientist, Department of Oncology and Hygiene, Tokyo Medical and Dental University, Japan

1999-2002 Postdoctoral Research Fellow, Institute for Molecular and Human Genetics, Georgetown University

2002-present Research Associate, Department of Medicine, Georgetown University

Award:

Kyoto Medical Society of International Student Fellowship, Japan (1990-1993)

Japanese Culture Ministry of International Student Fellowship, Japan (1989-1990)

Distinguished Student Award for Top Class Ranking, Sun Yat-sen University of Medical Sciences (1978, 1979, 1980, 1981 and 1982)

Scientific Societies:

The Japanese Human Genetics Association

Japanese Cancer Association

Japanese Molecular Biology Association

Chinese Biopharmaceutical Association

American Association for the Advancement of Science

American Heart Association

Publications:

1. Tamaki K., Horie K., Go T., Okuno T., Mikawa H., Zhang Y., Abe T., Sotos syndrome with a balanced reciprocal translation t (2;12)(q33.3;q15), Ann. Genet., 32: 244-246, 1992.

2. Zhang Y., Sugiura F., Abe T., Prenatal chromosome analysis on pregnancy women with polyhydramnios, J. Kyoto Medical Society, 39: 115-118, 1992.

3. Zhang Y., Sugiura F., Abe T., Rapid detection of sex chromosome aneuploides using fluorescent in situ hybridization (FISH), J. Kyoto Pref. Univ. Med., 102: 421-426, 1993.

4. Zhang Y., Prenatal Cytogenetic Study on 153 High Risk Pregnancies, J. Kyoto Pref. Univ. Med., 102,61-72, 1993.

5. Su Y-A., Bittner M.L., Chen Y., Tao L., Jiang Y., Zhang Y., Stephan D.A., TrentJ.M., Identification of Tumor Suppressor Genes using Human Melanoma Cell Lines UACC903, UACC903 (+6), and SRS3 by comparison of expression profiles, Mol. Carcino. 28 (2): 119-27, 2000.

6. Naimuddin M., Kurazono T., Zhang Y., Watanabe T., Yamaguchi M. and Nishigaki K., Species Identification Dots: A Potent Tool for Developing Genome Microbiology. Gene 261 (2): 243-250, 2000.

7. Wong L-J. C., Wang J., Zhang Y., Hsu E., Heim R.A., Bowman C.M., Woo M., Improved Mutation Detection of CFTR in Southern California Hispanic CF Patients, Hum. Mutat. 18(4): 296-307, 2001.

8. Ji H., Zhang Y., Zheng W. and Sandberg K., Altered Splicing of the
Angiotensin AT1A Receptor Correlates with Increased Renal AT1 Receptor
Expression in the Hypertensive Rat, Hypertension, submitted, 2004.

9. Zhang Y., Ji H., Zheng W., and Sandberg K., Translational Control of the Rat Angiotensin Type 1A Receptor by Alternative Splicing, Gene. Submitted, Review, 2004.

10. Ji H., Zhang Y., Zheng W. and Sandberg K., Translational Regulation of Angiotensin Type 1A Receptor Expression and Signaling by Upstream AUGS in the 5’ Leader Sequence, JBC, submitted, 2004.

11. Iafrati M.D., Zhang Y., Vitseva O., Deak L., Loscalzo J., and Freedman J.E., Decreased Arterial Thrombosis in Female Mice Deficient in Glutathione Peroxidase-1, 2004. In preparation.

Gene Cloning:

Zhang Y., Wong L-J. C., [2942insT]. It was found in a patient of Caucasian origin with cystic fibrosis. Cystic Fibrosis Genetic Analysis Consortium CFTR Mutation Table.

Web site: http://www.gent.sickkids.on.ca/cftr-cgi-bin/

Zhang Y., Wong L-J. C., [Q179K]. It was found in a Hispanic cystic fibrosis patient. Cystic Fibrosis Genetic Analysis Consortium CFTR Mutation Table.

Web site: http://www.gent.sickkids.on.ca/cftr-cgi-bin/

Zhang Y., Wong L-J. C., [3171delC]. It was found in a Hispanic cystic fibrosis patient. Cystic Fibrosis Genetic Analysis Consortium CFTR Mutation Table.

Web site: http://www.gent.sickkids.on.ca/cftr-cgi-bin/

Zhang Y., Freedman J.E., Human Megakaryocyte Endothelial Nitric Oxide Synthase gene promoter region and exon 1, partial cds, Access No. AF387340, Genebank.

Web site: http://www.ncbi.nlm.nih.gov/

Zhang Y., Freedman J.E., Human platelet Endothelial Nitric Oxide Synthase gene cDNA complete cds. Access No. AF400594, GeneBank

Web site: http://www.ncbi.nlm.nih.gov/

Abstracts:

1. Zhang Y., Sugiura F., Abe T., Chromosome analysis of amniotic fluids by in situ method, International Association of Human Biologists Conference on Isolation and Migration and Japan Society of Human Genetics, July, 1990, Fukui, Japan.

2. Zhang Y., Sugiura F., Abe T., Prenatal diagnosis of fetus with X chromosome abnormalities by fluorecent in situ hybridization (FISH), First Chinese-Japanese Human Genetics Symposium, October, 1992, Beijing, China.

3. Akiyama Y., Zhang Y., Yuasa Y., Analysis of CA repeats in HNPCC and sporadic tumors, Japanese Association for Molecular Biology Society, 16th Annual Meeting, December 16-19, 1993, Kyoto, Japan.

4. Akiyama Y., Zhang Y., Yuasa Y., Analysis of hMSH mutations in hereditary cancer, Japanese Association for Cancer Research, 53rd Annual Meeting, October, 1994, Nagoya, Japan.

5. Zhang Y., Sato T., Sato S., Maruyama I., Yuasa Y., Downward capillary transfer for DNA blotting, Japanese Association for Molecular Biology Society, 16th Annual Meeting, December 13-16, 1994, Kobe, Japan.

6. Zhang Y., Araki M., Sato S., Nishigaki K., TGGE and SSCP: A Comparison of two methods in detection of point mutation in DNA sequences, Japanese Association for Cancer Research, 56th Annual Meeting, September, 1997, Kyoto, Japan.

7. Zhang Y., Chakrabarti S., Freedman JE., A Novel Insertion Variant in the Promoter Region of Endothelial NO Synthase is Associated with Decreased Platelet-Derived NO and Promoter Activity in Megakaryocytes, Circulation, 2002; 106: 11-79 (A), USA

8. Zhang Y., Ji H., Zheng W., Sandberg K., Functionally distinct alternatively spliced angiotensin AT_1a receptor transcripts, Experimental Biology 2003 Annual Meeting, April 11-15, San Diego, CA, USA.

9. Ji H., Zhang Y., Zheng W., Sandberg K., Regulation of the Angiotensin AT1a Receptor by Alternative Splicing in Hypertension rats. XVth Scientific Meeting of the Inter-American Society of Hypertension. April 27-30, 2003 San Antonio, TX

10. Hassan A., Kim JW., Ji H., Zhang Y., Sandberg K., siRNA-mediated Silencing of an Angiotensin Type 1A Receptor Splice Variant in Vascular Smooth Cells. 57^th Annual Fall Conference and Scientific Sessions of the Council for High Blood Pressure Research in Association with the Council on the Kidney in Cardiovascular Disease. September 23-26, 2003, Washington DC, USA.

11. Iafrati MI, Zhang Y., Vitseva O, Loscalzo J, Freedman JE. Decreased arterial thrombosis in female mice deficient in cellular glutathione peroxidase-1. Circulation. 2003;108:IV-20.

12. Iafrati M.D., Zhang Y., Deak L., Koo E., Vitseva O., Freedman J E., Compensatory Mechanisms Influence Hemostasis in eNOS-Deficient Mice. Circulation. 2003;108:IV-256.

13. Welch JW., Solis G., Chabrashvili T., Zhang Y., Sandberg K., Xie H., Dennehy K., Aslam S., Marklund S., Wilcox C.,The roles of renal cuperoxide dismutases (SOD) on the slow pressor effects of angiotensin II.

14. Hassan A., Ji H., Zhang Y., Sandberg K., Splicing variant-specific RNA interference of angiotensin type 1a receptor (AT_1aR) demonstrates that AT_1aR expression is translationlly controlled by alternative splicing in rat aortic smooth muscle cells (RASMC). Experimental Biology 2004. Annual Meeting, April. Washingtong DC, USA.

15. Chen Y., Solis G., Zhang Y., Welch W.J., Wilcox C.S. Superoxide anion impairs endothelial function and vasodilatory propogation in cremaster arterioles of ECSOD-deficient and angiotensin II infused mice. Experimental Biology 2004. Annual Meeting, April. Washingtong DC, USA.